Summary
Type: SPE
Application #: 302270
Catalog#: 730012 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 200mg, 50/pk
Detection: HPLC, LC-MS
Conditions: Column conditioning: with two times 3 ml methanol and 3 ml distilled water.
Column washing: consecutively with 3 ml of distilled water, 3 ml of NaHCO3 (5%) and 3 ml distilled water.
Evaporation: by passing air through for 10 min and centrifuging at 4000units/min for 15 min.
Elution: with 2 ml acetone/dichloromethane (3:1, v/v).
Evaporation: to dryness under a stream of nitrogen at 60°C, and then reconstituted in 50 μL of methanol.
Authors: Hempel J Pforte H Raab B Engst W Böhm H Jacobash G
Source: Nahrung 43 (1999) Nr 3 S 201-204